Frédéric Bouché

Ph.D. in Plant Molecular Biology | Postdoctoral researcher | UW-Madison

Welcome!

I’m a plant molecular biologist at the University of Madison-Wisconsin. After completing my master degree in Biochemistry, Molecular and Cellular Biology at the University of Liège (Belgium), I started a Ph.D. thesis in the lab of Prof. Claire Périlleux, where I worked on the model plant species Arabidopsis thaliana. As an Aspirant F.R.S.-FNRS, I studied the genetic mechanisms controlling the flowering time at the whole-plant scale, including roots, and earned a Ph.D. degree in 2015. In parallel to my bench work, I initiated the development of FLOR-ID, a curated and interactive database summarizing the accumulated knowledge about the onset of reproductive development in Arabidopsis thaliana. As a Postdoc at the University of Wisconsin-Madison (BAEF Fellow), I am currently working with Prof. Richard Amasino, one of the leading expert in the field of the genetic control of plant reproductive development. I study the genetic pathways regulating the onset of flowering in Brachypodium distachyon, a model grass species closely related to wheat, barley, and oats.

Contact

fbouche@wisc.edu

Amasino lab
HF DeLuca Biochemistry Laboratories
University of Wisconsin-Madison
433 Babcock Drive
53706 Madison | United-States

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Projects

Understanding the mechanisms controlling flowering in Brachypodium distachyon

Brachypodium distachyon is a grass species from the Triticeae tribe, which includes wheat, barley, oat, and rye. A better understanding of the molecular mechanisms controlling the initiation of flowering in these species is necessary to improve yields. However, the economically important grasses tend to be physically large, have relatively long life cycles and possess complex genomes, thus making genetic studies very complex. In this context, Brachypodium emerges as a model organism to study the molecular mechanisms governing flowering in those species. The knowledge accumulated on the control of flowering time in Brachypodium will help researchers to better select inbred cereal crops.

Building a database of flowering time genes in Arabidopsis

The transition to flowering is an essential step of the plant life cycle that is tightly controlled by both endogenous and environmental cues. Its regulation is extremely complex and involves hundreds of genes that are part of highly interconnected pathways. Our knowledge of the molecular mechanisms governing the floral induction of Arabidopsis thaliana increases quickly and a significant number of reviews are published every year on this topic. Nevertheless, we believe that the current landscape of flowering-time research in Arabidopsis misses an exhaustive repository of the genes involved in the control of flowering and their regulatory pathways. Hence, we developed a web-based interactive resource organized around a curated database of the flowering time genes, available at www.flor-id.org. This project is a collaboration with Guillaume Lobet, and was published in Nucleic Acids Research.

Read more about it: Flowering highlights - F1000 Prime - ULg website - External Diffusion - Video description

Unravelling the shoot-root relationship in the context of flowering induction in Arabidopsis

The induction of flowering is a complex process involving hundreds of genes that are part of different interconnected pathways. Together, they converge to regulate a small subset of genes that are responsible for the switch from the vegetative to the reproductive phase of the plant life cycle. Photoperiodic induction of flowering is mediated by a mobile signal produced in phloem’s companion cells that subsequently migrates to the shoot apical meristem and likely reaches roots. My work intends to use an integrative approach to unravel the relationships that exist between roots and shoot during the induction of flowering but also to identify the role of flowering time genes expressed in roots.

Publications

Peer-reviewed articles

Integrating roots into a whole plant network of flowering time genes in Arabidopsis thaliana - 2016
Bouché F., D’Aloia M., Tocquin P., Lobet G., Detry N., & Périlleux C.
Scientific Reports, In press [URL]

FLOR-ID - an interactive database of flowering-time gene networks in Arabidopsis thaliana. - 2016
Bouché F., Lobet, G., Tocquin, P., & Périlleux C. & Tocquin P.
Nucleic Acids Research, 44 (D1) [URL]

Extracellular peptidase hunting for improvement of protein production in plant cells and roots. - 2015
Lallemand J., Bouché F., Desiron C., Stautemans J., de Lemos Esteves F, Périlleux C., & Tocquin P.
Frontiers in Plant Science, 6 [URL]

Heat can erase epigenetic marks of vernalization in Arabidopsis - 2015
Bouché F. , Detry N., & Périlleux C.
Plant Signalling and Behavior, 10 [URL]

A root chicory MADS-box sequence and the Arabidopsis flowering repressor FLC share common features that suggest conserved function in vernalization and devernalization responses. - 2013
Périlleux C., Pieltain A., Jacquemin G., Bouché F., Detry N., D’Aloia M., Thiry L., Aljochim P., Delansnay M., Mathieu A.-S., Lutts S., & Tocquin P.
The Plant Journal, 75 [URL]

Cytokinin promotes flowering of Arabidopsis via transcriptional activation of the FT paralogue TSF. - 2011
D’Aloia M., Bonhomme D., Bouché F., Tamseddak K., Ormenese S., Torti S., Coupland G., & Périlleux C.
The plant journal, 65 [URL]

Posters

Genetic Control of Flowering Time in Arabidopsis - an Interactive Database. 2015
Bouché F., Lobet G., Tocquin P., & Périlleux C.
Plant Organ Growth Symposium 2015, Ghent [Belgium]

Flowering Goes Underground. 2014
Bouché F., Lobet G., D’Aloia M., Detry N., Tocquin P., & Périlleux C.
The Bioforum 2014 of BioLiège, Liège [Belgium]

The hidden half of flowering. 2013
Bouché F., Mistiaen K., D’Aloia M., Detry N., Tocquin P., & Périlleux C.
Workshop on Molecular Mechanisms controlling flower development, Giens [France]

Molecular analysis of root medium impact on Arabidopsis thaliana development. 2013
Bouché F., André J., Tocquin P., & Périlleux C.
The Bioforum 2013 of BioLiège, Liège [Belgium]

A cytokinin route to flowering in Arabidopsis. 2011
Bouché F., André J., D’Aloia M., Bonhomme D., & Périlleux C.
Workshop on Molecular Mechanisms controlling flower development, Maratea [Italy]

Awards

Best Plant Biotechnology poster at the BioLiège Bioforum 2014. 2014
Prize awarded for the poster “Flowering Goes Underground”.

Presentations | Figshare profile

More about me

Work

Postdoc in Plant Genetics - Flowering-time control of Brachypodium distachyon [BAEF fellow] | Laboratory of Prof. Amasino, Dpt. of Biochemsitry, Univerity of Wisconsin-Madison (WI) | Dec. 2015-present

Training of 9 students | 3 Master II thesis - 6 Master I students | Laboratory of Plant Physiology, Phytosystems, University of Liège

Education

Ph.D. in Plant Genetics - Flowering-time control of Arabidopsis thaliana [Aspirant F.R.S.-FNRS] | Laboratory of Plant Physiology, Phytosystems, University of Liège | 2010-2015

Master in Biochemsitry, Molecular and Cellular Biology, University of Liège, Belgium | University of Liège, Belgium | 2008-2010

Bachelor in Biological Sciences | University of Liège, Belgium | 2006-2008

Personal

Member of the organizing board of the International Comblain Jazz Festival.

Music lover, photography enthusiast.